普鲁士蓝
化学
电化学
生物传感器
细胞培养
纳米技术
电化学电池
体内
三维细胞培养
电化学气体传感器
生物物理学
电极
细胞
组合化学
生物化学
材料科学
生物技术
物理化学
生物
遗传学
作者
Chun-Ying Yao,Yu Qin,Wen‐Ting Fan,Liping Yan,Ming Chen,Yanling Liu,Wei‐Hua Huang
标识
DOI:10.1016/j.jelechem.2022.116338
摘要
Three-dimensional (3D) cell culture has attracted increasing interest in many fields with the significant advantages in reproducing in vivo cell environment. For the characterization of biochemical information, electrochemical sensing has stood out from other techniques in real-time monitoring of living cells, and made an attempt to integrate with hydrogel for investigating the biological processes in 3D cell culture. However, it is still a challenge for real-time monitoring of cellular metabolites therein for the lack of electrochemical activity, and lactate (the product of aerobic glycolysis) is a typical example of such a predicament. Herein, we developed a 3D enzymatic electrochemical sensor that could be integrated with collagen hydrogel to monitor the lactate levels of cells cultured therein. Specifically, lactate oxidase immobilized on the outer surface of 3D sensor could oxidize lactate with H2O2 as a product, and Prussian blue nanoparticles electrodeposited on carbon nanotubes adsorbed electrode conferred the excellent and stable electrocatalytic performance to H2O2 for lactate detection. Further, the 3D sensor was integrated with collagen hydrogel with C6 glioma cells seeded therein and allowed the successful real-time monitoring of lactate released from C6 glioma cells, providing an effective detection platform for exploring the biochemical response in 3D cell culture systems.
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