Explore the mechanism of pulsed electric field technology on improving the antioxidant activity of Leu-Tyr-Gly-Ala-Leu-Gly-Leu

In this study, the synthetic peptide Leu-Tyr-Gly-Ala-Leu-Gly-Leu (LYGALGL) derived from egg white was used as the research object. The structure - activity relationship was elucidated by cell experiment and structure determination. The results of cell experiment showed that the superoxide dismutase (SOD) activity and mitochondrial membrane potential (MMP) of LYGALGL were significantly increased by pulsed electric field (PEF) treatment, especially by 10 kV/cm field intensity treatment ( P < 0.05). However, the content of reactive oxygen species (ROS) decreased significantly ( P < 0.05). LYGALG treated with 10 kV/cm showed the highest antioxidant activity. The results of structure determination showed that the β-turn of LYGALGL increased, the random coil decreased, and the active hydrogen sites moved under 10 kV/cm treatment. The structure of LYGALGL was changed and the active site was exposed by PEF, thus improving the antioxidant activity of LYGALGL.