Protocols for Immunofluorescence Techniques

Since the initial description of immunofluorescence (IF) assay by Coons, Creech, and Jones in 1941, it has evolved from being an academic technique to a routine laboratory investigative practice. Immunofluorescence technique is a very useful tool to detect protein expression and localize cellular as well as viral antigen (in case of virus infected cell). Immunofluorescence technique detects the target optically using fluorophore conjugated-antibodies. In this chapter, we have described various types of IF techniques, the advantages and limitations associated with each variant. Here we also describe the protocol for performing immunofluorescence technique along with the important points to note and alternative ways for each step involved while performing the IF assay.