仿形(计算机编程)
RNA序列
计算生物学
生物
抄写(语言学)
转录因子
发起人
基因表达谱
基因组
基因
计算机科学
基因表达
遗传学
转录组
语言学
哲学
操作系统
作者
Robert A. Policastro,Gabriel E. Zentner
出处
期刊:Springer US eBooks
[Springer Nature]
日期:2022-01-01
卷期号:: 21-34
被引量:2
标识
DOI:10.1007/978-1-0716-2257-5_2
摘要
Transcription start site (TSS) usage is a critical factor in the regulation of gene expression. A number of methods for global TSS mapping have been developed, but barriers of expense, technical difficulty, time, and/or cost have limited their broader adoption. To address these issues, we developed Survey of TRanscription Initiation at Promoter Elements with high-throughput sequencing (STRIPE-seq). Requiring only three enzymatic steps with intervening bead cleanups, a STRIPE-seq library can be prepared from as little as 50 ng total RNA in ~5 h at a cost of ~$12 (US). In addition to profiling TSS usage, STRIPE-seq provides information on transcript levels that can be used for differential expression analysis. Thanks to its simplicity and low cost, we envision that STRIPE-seq could be employed by any molecular biology laboratory interested in profiling transcription initiation.
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