Epitope-imprinted polymers: applications in protein recognition and separation

Molecularly imprinted polymers (MIPs) have evolved as promising platforms for specific recognition of proteins. However, molecular imprinting of the whole protein molecule is complicated by its large size, conformational instability, and structural complexity. These inherent limitations can be overcome by using epitope imprinting. Significant breakthroughs in the synthesis and application of epitope-imprinted polymers (EIPs) have been achieved and reported. This review highlights recent advances in epitope imprinting, from the selection of epitope peptide sequences and functional monomers to the methods applied in polymerization and template removal. Technological innovations in detection and extraction of proteins by EIPs are also provided.