Apocynin derivatives as potential inhibitors of NADPH oxidase

NADPH oxidase (nicotinamide adenine dinucleotide phosphate oxidase), is the major source of superoxide anion (O2.‐), wich is a free radical and reacts with Nitric Oxide (NO) decreasing its bioavailability in endothelial cells producing severe cardiovascular diseases such as hypertension. It had been reported that Apocynin inhibit NADPH oxidase, however, has not been used as a therapeutic treatment, due to Apocynin needs to be converts to its dimeric form by Mieloperoxidasa (MPO) and this enzyme has low affinity for Apocynin. Therefore, in this work were synthesized a set of Apocynin ethers with the main to compounds as obtain better NADPH oxidase inhibitor than Apocynin and avoiding the MPO dimerization. The inhibitory activity of the compounds was evaluated by Electron Paramagnetic Resonance (EPR) employed homogenized of aortic tissue contained NADPH oxidase of spontaneously hypertensive (SHR/NHsd) and normotensive (WKY/NHsd) rats.