DPPH
化学
抗氧化剂
EC50型
抗氧化能力
有机化学
色谱法
生物化学
体外
作者
Krishnanand Mishra,Himanshu Ojha,N. K. Chaudhury
出处
期刊:Food Chemistry
[Elsevier]
日期:2012-02-01
卷期号:130 (4): 1036-1043
被引量:767
标识
DOI:10.1016/j.foodchem.2011.07.127
摘要
Applications of antioxidants are increasing due to their multiple roles in minimising harmful effects of oxidative stress. 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay is routinely practiced for the assessment of antiradical properties of different compounds. A detailed literature survey revealed use of different materials and methods for DPPH assay by different investigators resulted in variation in the values of reference standards and measured parameters of new antioxidants. In the present work a detailed kinetic study of antioxidants has been performed and comprehensive results in terms of effective concentration which scavenges 50% radical (EC50), antioxidant reducing power (ARP), stoichiometry and second order rate constant (k2) values have been reported with DPPH assay. Importance of selection of appropriate reference compounds and kinetic calculations are suggested. Few case studies of standard antioxidants have been discussed to emphasise the utilisation of appropriate methodology and reference compounds.
科研通智能强力驱动
Strongly Powered by AbleSci AI