纳米孔
生物分子
纳米技术
电化学
电解质
安培法
化学
材料科学
电极
物理化学
作者
Xinchun Li,Tianchi Zhang,Pengcheng Gao,Benmei Wei,Yi‐Xia Jia,Yong Cheng,Xiaoding Lou,Fan Xia
出处
期刊:Langmuir
[American Chemical Society]
日期:2018-08-21
卷期号:34 (49): 14787-14795
被引量:20
标识
DOI:10.1021/acs.langmuir.8b02010
摘要
Nanopore ionic current measurement is currently a prevailing readout and offers considerable opportunities for bioassays. Extending conventional electrochemistry to nanoscale space, albeit noteworthy, remains challenging. Here, we report a versatile electrochemistry array established on a nanofluidic platform by controllably depositing gold layers on the two outer sides of anodic aluminum oxide (AAO) nanopores, leading to form an electrochemical microdevice capable of performing amperometry in a label-free manner. Electroactive species ferricyanide ions passing through gold-decorated nanopores act as electrochemical indicator to generate electrolytic current signal. The electroactive species flux that dominates current signal response is closely related to the nanopore permeability. Such well-characteristic electrolytic current-species flux correlation lays a premise for quantitative electrochemical analysis. As a proof-of-concept demonstration, we preliminarily verify the analytical utility by detection of nucleic acid and protein at picomolar concentration levels. Universal surface modification and molecule assembly, specific target recognition and reliable signal output in nanopore enable direct electrochemical detection of biomolecules without the need of cumbersome probe labeling and signal amplification.
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