Biocompatibility of Rappid Promotyping PLGA/TCP Tissue Engineered Bone Scaffolds Coated with Collagen in vitro

The goal of present study is to investigate the biocompatibility of rapid prototyping(RP) polylactic glycolic acid/tricalcium phosphate(PLGA/TCP) scaffold coating with collagenⅠ and provide a basis for in vivo experiments.PLGA/TCP scaffold fabricated with RP technology and coated with collagen I was used as experimental group and original scaffold without collagenⅠ was used as control group.After water absorption determined,BMSCs were seeded into both groups.Adhesion and proliferation ratio were examined.Morphology of BMSCs were observed under scanning electron microscope(SEM).Higher hydrophilicity was performed in experimental group.Compared with control group,the Cell adhesion ratio was increased significantly in the experimental group at 4,8,and 12 hour after the cell seeding(P0.05).As well,the cell proliferation of experimental group was demonstrated at higher level at 2,4,6,and 8 days(P0.05).In addition,the quantity of BMSCs in experimental group was much more than control group under SEM.It is conclused this rapid prototyping PLGA/TCP scaffold coated with collagenⅠ possesses good cellular biocompatibility.It can be used as a scaffold to restore bone defects in bone tissue engineering.