Isolation and Identification of Botrytis cinerea from Dendrobium devonianum

For understanding the relationship between Dendrobium devonianum and fungal on it,tissue isolation were conducted for root,stem and leaf in both diseased and healthy Dendrobium devonianum,and a total 306 fungal strains were obtained. Among these fungal strains,the largest number was Botrytis cinerea,which accounted for 23. 4 %. After purified culturing,the Botrytis cinerea were identified by means of combining morphological characters with molecular biological techniques. Based on the the fact of host range and distribution range of Botrytis cinerea,this study showed that D. devonianum is a new host for Botrytis cinerea and this was the first report that D. devonianum could be host of Botrytis cinerea.