流式细胞术
细胞周期
细胞生物学
小区同步
同步(交流)
DNA
细胞
细胞仪
计算生物学
生物
计算机科学
生物系统
分子生物学
遗传学
频道(广播)
计算机网络
作者
Naoko Yoshizawa-Sugata,Hisao Masai
出处
期刊:Methods in molecular biology
日期:2014-01-01
卷期号:: 279-293
被引量:9
标识
DOI:10.1007/978-1-4939-0888-2_13
摘要
Analysis of cellular DNA content and measurement of pulse-labeled newly replicated DNA by flow cytometry are useful techniques for cell cycle studies. In this chapter, we describe the protocols for cell cycle synchronization of mammalian cells, including time course designs and consideration of cell types to achieve successful experiments, along with the methods for detection of DNA. Some selected applications dealing with siRNA-mediated knockdown are also presented.
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