STUDIES ON POLYPLOID INDUCTION BY COLCHICINE IN PINUS BUNGEANA

Cotyledon explants of mature embryos of Pinus bungeana tissue were culture,the use of colchine induced polyploid.Cotyledons were cultured for 10 days,with the quality of volume fraction of 0.01%,0.03%,0.05% of colchicine immersion,respectively 6 h,12 h,24 h,observation of cotyledon growth and bud differentiation status,identification of chromosome doubling in 20 days.Results indicated that:different concentrations of colchicine effectfed cotyledon growth effect.Concertration was higher,processing time was longer,the higher the death;colchicine treated shoot regeneration is slow,and shoot regeneration rate increased with the concentration decreased;colchicine treated cotyledons chromosomes have doubled,but with colchicine concentration enhancement and treatment the extension of time,polyploid induction rate showed a downward trend.Data displayed quality score of 0.03% for the combination of 12 h treated with colchicine,polyploid induction rate was highest,40.5%.The study on polyploid induction of Pinus bungeana system were initially established.