Brucelosis is an emerging zoonotic disease in many countries around the world. There are some reports of Brucella abortus infections in cattle and humans in Ecuador, nevertheless, other Brucella species have not been identified. This study was designed to identify circulating Brucella species in 300 goat samples and one canine fetus from 8 different provinces of the highland Andes of the country. The results showed isolates from Brucella melitensis, Brucella suis, Brucella abortus y Brucella canis.