适体
表面增强拉曼光谱
检出限
表面等离子共振
生物传感器
分析物
基质(水族馆)
纳米技术
癌症生物标志物
材料科学
癌症
纳米颗粒
色谱法
化学
分子生物学
拉曼光谱
生物
拉曼散射
光学
生态学
物理
遗传学
作者
Penghui Shao,Danyang Dong,Liping Zhong,Qianqian Wang,Pan Lu,Qunshan Zhu,Zheng Wang,Fengjuan Jiang,Wei Wei
摘要
Gastric cancer (GC) as is the second deadliest malignancy still lacks rapid, simple and economical detection and early clinical screening techniques. Surface-enhanced Raman spectroscopy (SERS) is a spectroscopic technique based on the surface plasmon resonance of precious metal nanoparticles, which can effectively detect low-abundance tumor markers. Combining SERS technology with sensors has high potential in the diagnosis and screening of GC.A novel Au/Si nano-umbrella array (Au/SiNUA) was prepared as a SERS substrate and the substrate was functionalized using the corresponding tumor marker aptamers for the detection of clinical biological samples using a one-step recognition release mechanism. Optimization of aptamer and complementary chain concentrations and detection time for optimal sensor preparation.Au/SiNUA were tested to have good SERS enhancement activity. The proposed aptamer biosensor has good specificity and stability, with a low detection time of 18 min and a limit of detection (LOD) at the fM level, which is superior to most of the methods reported so far; and the accuracy of the clinical assay is comparable to that of the ELISA method. The expression levels of PDGF-B and thrombin in the serum of GC patients and healthy individuals can be effectively detected and differentiated.The ultrasensitive and specific aptamer biosensor is highly feasible for the diagnosis and screening of GC and has good application prospects.
科研通智能强力驱动
Strongly Powered by AbleSci AI