蛋白酵素
蛋白酶
鞣花酸
熊果酸
化学
传统医学
HIV-1蛋白酶
药理学
色谱法
生物化学
酶
生物
医学
多酚
抗氧化剂
作者
Yong Zhou,Sumei Li,Xin Wei,Xin Yang,Jun Xiao,Bo Wen Pan,Shou Xia Xie,Ying Zhou,Jian Yang,Ying Wei
标识
DOI:10.1080/14786419.2022.2162513
摘要
Potentilla kleiniana Wight et Arn(PK, 'Wu Pi Feng' in Chinese) was recorded as Miao ethnic medicine for treatment of fever, cough, ulcer, and erysipelas for thousands years. This study aimed to evaluate the antiviral activity of four PK extracts and seven compounds by using HIV-1 protease (HIV-1 PR). In addition, Ultra-High Performance Liquid Chromatography and High Resolution Mass Spectrometry (UPLC-HRMS) was employed to identify the bioactive components. The toxicity assessment of the extracts was done before antiviral screening using a highly specific human aspartyl protease, renin protease by fluorimetric method. As a result, seven compounds and four extracts of PK inhibited HIV-1 PR with IC50 range from 0.009 to 0.36 mg/mL, and did not appreciably inhibit the general human protease renin. This study first demonstrated that four PK extracts, ellagic acid and ursolic acid potent inhibit HIV-1 protease, could be used as an efficacious drug candidate to treat SARS-CoV-2 infection.
科研通智能强力驱动
Strongly Powered by AbleSci AI