表面等离子共振
生物分子
生物传感器
等离子体子
DNA
共振(粒子物理)
材料科学
表面等离子体子
分析化学(期刊)
电介质
光纤传感器
纤维
化学
纳米技术
光电子学
物理
色谱法
纳米颗粒
生物化学
粒子物理学
复合材料
作者
F. H. Suhailin,Ali Abdulkhaleq Alwahib,Hui Yee Chee,Fatin Hamimi Mustafa,Amir Syahir,Nurul Hida Zainuddin,Muhammad Firdaus Omar,Anis A.A. Razak,Mohd Adzir Mahdi
出处
期刊:IEEE Sensors Journal
[Institute of Electrical and Electronics Engineers]
日期:2022-11-01
卷期号:22 (21): 20516-20523
被引量:2
标识
DOI:10.1109/jsen.2022.3207498
摘要
Fiber-based surface plasmon resonance (SPR) sensors are the ideal configuration for rapid and label-free biomolecule detection. Gold-coated tapered optical fiber was used to excite the SPR mode that propagates at the metal–dielectric interface. By forming self-assembled monolayers (SAMs) of active chemical functional groups, the deoxyribonucleic acid (DNA) probe targeting the secY gene of Leptospira was immobilized onto the sensor surface. Red shifts in the SPR wavelengths were observed at different concentrations of leptospiral DNA target against the immobilized DNA probe due to the ability of plasmonic interaction to sensitively detect the binding of biomolecules on the sensor area. The developed sensor showed satisfactory linear responses at two target concentration ranges; low (1.0 fM to 1.0 pM) and high (0.1 nM to $1.0~\mu \text{M}$ ), with the highest sensitivity of 1.22 nm per log M. The negative control test also revealed high sensor specificity toward the complementary DNA strand. This sensor will represent a significant step toward laboratory-free diagnosis of leptospirosis.
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