A targeted nanozyme based on multiple porphyrins for enhanced photodynamic antibacterial application

High H2O2 content at the bacterial infection site causes irreversible damage to DNA, RNA and proteins, and influences intracellular signaling through essential signaling proteins that control cell proliferation. Catalase can decompose H2O2 to produce O2 in situ, which is an ideal strategy to reduce H2O2 in the infectious tissue. In this work, a novel targeted nanozyme CoⅡTBPP(bpy) is precisely synthesized by utilizing supramolecular self-assembly. The prepared nanozyme exhibits excellent ability to capture bacteria due to its phenylboronic acid groups and has broad-spectrum antibacterial efficiency through the synergy of PDT, the change of membrane permeability and the peroxidase-like •OH production. In addition, CoⅡTBPP(bpy) has significant effect on anti-inflammatory and promoting wound healing, which is proved in E. coli-infected rat ulcers model in vivo and shows very low cytotoxicity to normal mammalian cells. With further development and optimization, as a new type of antibacterial nanozyme, CoⅡTBPP(bpy) has a great potential for the treatment of wound infections.