弗氏柠檬酸杆菌
生物转化
聚合物
色谱法
实验室烧瓶
甘油
明胶
化学
聚苯乙烯
生物反应器
化学工程
有机化学
生物化学
工程类
基因
大肠杆菌
肺炎克雷伯菌
发酵
作者
Michael Griffiths,John A. Bosley
标识
DOI:10.1016/0141-0229(93)90033-x
摘要
POLYHIPE polymers represent a novel immobilization support possessing many desirable characteristics. These materials have been used to immobilize Citrobacter freundii in order to develop a continuous bioconversion system for the anaerobic production of trimethylene glycol from glycerol. Several immobilization methods were employed, of which the most successful involved culturing the organism with POLYHIPE in shake-flasks, transferring the contents into a column, and growing the cells in situ within the column. Cell activity was determined by the amount of TMG produced from a buffered glycerol solution. The latter was passed through the columns by either a recirculation system or the superior single-pass method. The optimal system was scaled up and showed potential for further large-scale investigations. Cell lifetime in the column could be improved by supplying the cells with diluted growth medium in place of the buffer.
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