杆状病毒科
计算生物学
计算机科学
生物
重组DNA
九氟化硫
细胞生物学
苜蓿银纹夜蛾
基因
作者
Cheryl Isaac Murphy,Helen Piwnica-Worms
标识
DOI:10.1002/0471140864.ps0504s00
摘要
Baculoviruses have emerged as a popular system for overproducing recombinant proteins in eukaryotic cells. This unit gives an overview of the baculovirus expression system, including discussion of the baculovirus life cycle, and post-translational modifications that occur in insect cells. In addition, the steps for overproducing proteins in the baculovirus systems are described along with recommendations for choosing an appropriate baculovirus vector and DNA, and reagents and equipment necessary for implementing the whole overexpression system.
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