生物过程
生化工程
生产力
瞬态(计算机编程)
工作(物理)
计算机科学
计算生物学
风险分析(工程)
生物
工程类
医学
经济
宏观经济学
操作系统
机械工程
古生物学
作者
Jesús Lavado‐García,Pol Pérez‐Rubio,Laura Cervera,Francesc Gòdia
标识
DOI:10.1016/j.biotechadv.2022.108017
摘要
One of the main challenges in the development of bioprocesses based on cell transient expression is the commonly reported reduction of cell specific productivity at increasing cell densities. This is generally known as the cell density effect (CDE). Many efforts have been devoted to understanding the cell metabolic implications to this phenomenon in an attempt to design operational strategies to overcome it. A comprehensive analysis of the main studies regarding the CDE is provided in this work to better define the elements comprising its cause and impact. Then, examples of methodologies and approaches employed to achieve successful transient expression at high cell densities (HCD) are thoroughly reviewed. A critical assessment of the limitations of the reported studies in the understanding of the CDE is presented, covering the leading hypothesis of the molecular implications. The overall analysis of previous work on CDE may offer useful insights for further research into manufacturing of biologics.
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