生物发光
三磷酸腺苷
荧光素
荧光素酶
生物分析
纳米技术
化学
材料科学
生物化学
转染
基因
作者
Maria Maddalena Calabretta,Ruslán Álvarez-Diduk,Elisa Michelini,Arben Merkoçi
出处
期刊:Methods in molecular biology
日期:2022-01-01
卷期号:: 297-307
被引量:2
标识
DOI:10.1007/978-1-0716-2473-9_23
摘要
Adenosine-5'-triphosphate (ATP) is the primary energy carrier in all living organisms, and its detection in living cells represents a well-established approach. ATP-driven bioluminescence (BL) relying on the D-luciferin-luciferase reaction is a bioanalytical tool widely employed for monitoring hygiene and microbial contamination of foods.Here, we report a straightforward method for ATP BL detection using an ATP sensing paper fabricated with an alternative freeze-dry procedure. The assay can be easily implemented in laboratories equipped with (i) freeze-drying, wax printing, and 3D printing technologies and (ii) instrumentation for BL detection such as benchtop luminometers and portable light detectors including a smartphone camera without the need for additional equipment.
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