Study on Technology of Separation and Purification for Flavones in Lotus Leaf by HP-20

As the chief activity component,lotus flavoneshave beenone of the hot points of healthy products research.The research compared the effect of 5 kinds of resins of X-5,HP-20,NKA,AB-8 and HPD-100.The results showed that HP-20 was the best one.Its static absorbtion was 68.90 mg/g resin.The rate of desorption was 87.23%.The research optimized the technique parameters systematically of HP-20 separating lotus flavones.The results showed that the saturated absorption of lotus flavones by HP-20 was 15 BV of lotus flavone of 7 mg/mL concentration.The concentration of lotus flavones could reach 62.06% desorbed by 40% ethanol for one times when the concentration of the added lotus flavones was 200 mg/mL and the speed of desorption was 2 BV/h.The rate of lotus flavones desorbed by 40% ethanol was 81.40% and the total rate of desorbed lotus flavones was 98.17%.The concentration of lotus flavones can reach to 98.87% if they are separated for several times.