Effect of matrine on the proliferation of human pterygium fibroblasts

OBJECTIVE To study the influence of Matrine on the proliferation of cultured human pterygium fibroblasts(HPF).METHODS Colorimetry MTT was used to detect viability of Matrine at different concentrations(0.1,0.2,0.4,0.8,1.6,3.2,6.4 and 12.8 mg/ml)at different time(24,48 hours)for inhibition of proliferation of HPF.RESULTS Administration of 0.4～12.8 mg/ml Matrine for 24～48 h could significantly inhibit HPF proliferation in a dose dependent manner(P0.05).There was obvious inhibitory effects of Matrine on HPFs in the concentration of 0.4 mg/ml for 24 h(P0.05,the rate of inhibition is 22.93 %),and was more evident in the concentration of 0.4 mg/ml for 48 h(P0.01,the rate of inhibition is 30.59 %)as compared with control group.The 50 % inhibitory concentration(IC50)of Matrine was 1.8115 mg/ml.CONCLUSIONS There was a remarkable inhibitory effect of Matrine on HPFs,in a time-dependent and dose-dependent manner.