Experimental Research of the Protection of Centalla Asiatica Compound for Podocyte Injury in Focal Segmental Glomerulosclerosis Rats

Objective:To investigate the altered expression level of slit diaphragm (SD) protein in focal segmental glomerulosclerosis (FSGS) rats after being treated by centalla asiatica compound(CAC) and to illuminate the CAC’s effect of delaying glomerulosclerosis in molecular level. Methods:The FSGS rat models were made by unilateral nephrectomy plus adriamycin(ADR) injection twice.The CAC-treated rats were respectively treated with low、medium and high dosage of CAC unguentum while the control group was treated with benazepril suspension. At the end of the model-establishing process, the 8th week, the 24h urinary proteinthe and the serum levels of Scr,Bun,Tch,TG and Alb were detected. The cortex tissue in the right kidney was preserved to observe the pathological changes by HE staining through light microscope and to detect the expression of nephrin and podocin in mRNA and protein levels using respectively RT-PCR and immunohistochemistry methods. Results:Compared with the model rats, the levels of 24 h urinary protein、Scr、Bun、Tch and TG were improved significantly in all CAC-treated groups,and there was no marked difference between the CAC groups and the benazepril group. The result of RT-PCR and Immunohistochemistry showed that the nephrin and podocin expression was obviously slight in model group than that in normal group(P0.05).And the expression in all treatment groups were increased markedly in contrast with the model group(P0.05), but with no significant difference contrasted to the normal group(P0.05). Conclusion:The findings suggested that the CAC can protect the podocyte from being injuried and has the better efficiency of anti-glomerulosclerosis in FSGS model rats which probably is obtained through up-regulating the expression of nephrin and podocin .