A class of biocatalyst:cyanide-resistant nitrile hydratase

Cyanide resistant-nitrile hydratase was a class of biocatalyst.Compared with ordinary nitrile hydratase,it could tolerate high concentrations of the cyanide without inhibition,thus,it opened up a new pathway for the industrial synthesis of α-hydroxyamides and α-aminoamides.Study of the cyanide resistant mechanism of nitrile hydratase and the improvement of the tolerance capacity were the two main problems needed to be solved.The inhibition mechanism of the nitrile hydratase by the cyanide,as well as the discovery of cyanide-resistant nitrile hydratase and the applications in the synthesis of methionine and 2-hydroxyisobutyric acid amide were introduced.The basic ideas for researching cyanide resistant-nitrile hydratase were also discussed.