Physiological effects of NaCI on salt stress of lily plantlets from tissue culture

In oreer to explore the feasibility of lily salt tolerance evaluation under salt stress and mechanism with a view to the selection of salt-resistant varieties and provide a theoretical basis, the physiological index changes of lily plantlets from tissue culture with NaCl treatment were determined. By experiment, the threshold about salt tolerance of lily microplants was 8 g/L NaCl. The results showed that the content of Malondialdehyde (MDA) and the leakage of electrolytes increased when treated with 8 g/L NaCl,and the changes in leaves were more obvious than that in roots. Proline (Pro) content did not show a obvious regulation. The activities of antioxidant enzymes (SOD,POD,CAT and APX) increased under first period of salt stress and then decreased. The change was more obvious in leaves than in roots. Meanwhile,the content of ASA decreased and it changed more rapidly in leaves than in roots. It was concluded that the roots of lily had better salt tolerance than the leaves. MDA and the leakage of electrolytes could be used as stable indicators of the salt tolerance evaluation and Pro is not appropriate.