Study on immobilization of D-tagatose 3-epimerase

D-tagatose 3-epimerase(DTE)was immobilized on 14 kinds of ion-exchange and adsorption resins,in which D301-Ⅲ was the best material for the immobilization.Then the enzyme was immobilized by cross-linkage of glutaraldehyde.The optimum conditions for the immobilization were as follows:2.00mL/g(liquid enzyme/wet resin),pH7.5,immobilization temperature 30℃,and immobilization time 8h.The concentration of the crosslinking agent(glutaraldehyde)was 0.05%.The crosslinking time was 3h.The activity recovery yield of immobilized enzyme was above 50%.The remaining activity was more than 65% of the primary enzyme activity after the immobilized enzyme was used repeatedly for 10 times.It showed that the immobilized DTE had good operational stability.