Determination of Ginsenoside Rg_1,Ginsenoside Re and Ginsenoside Rb_1 in Naomai Shukang Capsule by HPLC

Objective:To estiblish an HPLC method for ginsenoside Rg1,ginsenoside Re and ginsenoside Rb1 in Naomai Shukang Capsule.Method:An Hypersil-C18 column(4.6 mm × 250 mm,5 μm) was used.The mobile phase was composed of acetonitrile(A) and 0.1% phosphoric acid(B) in gradient mode.The detection wavelength was 203 nm.Result: Ginsenoside Rg1,ginsenoside Re and ginsenoside Rb1 showed good linear relationship in the range of 0.210-1.680 mg·L-1(r = 0.999 96),0.522-4.176 mg·L-1(r = 0.999 98),1.092-7.644 mg·L-1(r = 0.999 9) respectively.The average recoveries of ginsenoside Rg1,ginsenoside Re and ginsenoside Rb1 were 97.01%(RSD 2.42%,n = 5),97.88%(RSD 1.48%,n = 5),96.19%(RSD 1.67%,n = 5) respectively.Conclusion:The method was accurate reliable,and specific,can be used for the quality control of Naomai Shukang Capsule.