Use of Filter Aided Sample Preparation to Study Milk Fat Globule Membrane Proteins

Bovine milk is consumed widely in the world.It provides multiple nutrients to human,including protein,fat,carbohydrate,vitamins and minerals.Protein is a major component of bovine milk.It contains caseins,whey protein and milk fat globule membrane protein(MFGM).Milk fat exists as milk fat globules in bovine milk and covered by a three-layer membrane which consists of lipids and protein.Recently,the identification of milk proteins gained interests.As the development of proteomic techniques,the number of identified milk protein is increasing.However,no fast and efficient method has been arisen to identify the MFGM proteins because of their hydrophobicity.Filter-aided sample preparation and NanoLC-MS/MS were combined and applied to study MFGM protein in present study.By using these methods,it is now possible to study MFGM in a fast and efficient way.