Studies on enriching factor of Lactobacillus rhamnosus L7-13 strain by response surface methodology

Response surface methodology was used to optimize the MRS-based enriching factor of Lactobacillus rhamnosus L7-13 strain.According to the determination of viable count,the enriching effects of several additives were evaluated by using the single-factor test.screen out the main affecting factors on the Lactobacillus rhamnosus L7-13 strain.Then the central composite design and response surface analysis were used to determine the optimal levels of the main factors.Cultived in the optimized medium,the cell numerous of Lactobacillus rhamnosus L7-13 Strain increased from 7.9×108cfu/mL to 8.1×1010cfu/mL.Experiments showed that this media and culture conditions were suitable for growth and reproduction of Lactobacillus rhamnosus L7-13 strain.