A simple electroanalytical method for estimation of Pioglitazone Hydrochloride and Gliclazide individually from pharmaceutical formulation.

A simple, specific, accurate, precise and reproducible method has been developed and validated for the Pioglitazone Hydrochloride and Gliclazide individually using Differential Pulse Polarography (DPP) technique. Quantification of Pioglitazone Hydrochloride and Gliclazide was done in Britton-Robinson Buffer pH 6.0 and pH 2.5 respectively using 1M KCl as a supporting electrolyte. Both Pioglitazone Hydrochloride and Gliclazide exhibit reduction cathodic peak in given respective pH with peak potential (Ep) as -1.62V for Pioglitazone Hydrochloride and -1.180V for Gliclazide vs. S.C.E. .The parameters were used for method validation are linearity; accuracy, precision, LOD and LOQ. Proposed method was successfully applied for routine quality control analysis and determination Pioglitazone Hydrochloride and Gliclazide individually in drug formulation.