Design, Synthesis, and Evaluation of Organic and Organometallic Pyrazoline Derivatives as Selective Dual COX-2/5-LOX Inhibitors and Potential Anticancer Agents

Inspired by the structure of the anti-inflammatory drug Celecoxib, which is currently used in cancer prevention and treatment, we report the design, synthesis, and biological evaluation of organic and organometallic molecular hybrids based on pyrazolines (4a-h). Structure-Activity Relationship (SAR) analyses showed that the combination of catechol-benzenesulfonamide in 4a (organic) and 4c (ferrocenyl) derivatives acts as potent and highly selective dual inhibitors (IC₅₀ COX-2 = 4.58 and 2.88 μM; IC₅₀ 5-LOX = 0.23 and 0.10 μM, respectively; evaluated against COX-1 and 15-LOX isoforms). Molecular dynamics simulations of 4a and 4c in 5-LOX showed their preferential localization at the allosteric site and at the entry channel, respectively, consistent with their noncompetitive (4a) and mixed (4c) kinetics. Furthermore, the noncytotoxic complex 4c (MRC-5, CC₅₀ = 38.13 μM) exhibited anticancer effects in ovarian cancer cells (A2780, CC₅₀ = 13.79 μM) that overexpress the proinflammatory enzymes COX-2 and 5-LOX (Western Blot), exceeding the activity of the drug Celecoxib.