Chiral Separations Using Native and Functionalized Cyclodextrin-Bonded Stationary Phases in High-Pressure Liquid Chromatography

Cyclodextrin bonded stationary phases for HPLC have had a pronounced effect in the area of chiral separations. They are hydrolytically stable and have demonstrated unusual selectivity in the reversed and normal phase modes. Their selectivity is in part due to the inclusion complexation properties of cyclodextrins. Native and derivatized cyclodextrin bonded stationary phases have shown versatility for chiral separations. They are discussed in terms of their retention and chiral recognition mechanisms. Structural-selectivity relationships were observed for analytes using these columns. Optimization parameters are reviewed.