Preparation, structural characterization and antioxidant activity of water-soluble polysaccharides and purified fractions from blackened jujube by an activity-oriented approach

This study aimed to investigate the structural features and antioxidant activity in vitro of crude blackened jujube polysaccharides (BJPs) and five purified polysaccharides based on the activity-oriented approach. The crude BJPs exhibited dose-dependent radical scavenging activity and total reducing capacity, and provided excellent protective effects against H2O2-damaged HUVECs via up-regulating mitochondrial membrane potential and down-regulating intracellular reactive oxygen species. After fractionation by column chromatography, the five purified components differed in chemical composition, molecular weight, monosaccharide composition (type and relative proportion) and FTIR band (peak pattern or intensity, especially in the range of 1000.0-1200.0 cm-1), as well as protective effects against H2O2-induced HUVECs. As the most abundant and potent antioxidant component, the backbone of BJP-3 was mainly composed of →4)-α-l-GalpA (1→, →5)-α-l-Araf (1→ residues with two terminals of T-α-l-Araf (1→ and T-β-d-Galp (1→. The above results compared the structural and bioactive properties of different blackened jujube polysaccharides and highlighted their potential as antioxidants for functional foods.