细胞外小泡
分离(微生物学)
真核生物
计算生物学
超离心机
核酸
表征(材料科学)
原核生物
微泡
纳米技术
细胞外
细胞生物学
化学
生物
生物物理学
生物化学
小RNA
材料科学
生物信息学
基因组
基因
作者
Chihchen Chen,Boren Lin,Min‐Yen Hsu,Chao‐Min Cheng
摘要
Extracellular vesicles (EVs), membranous particles released from various types of cells, hold a great potential for clinical applications. They contain nucleic acid and protein cargo and are increasingly recognized as a means of intercellular communication utilized by both eukaryote and prokaryote cells. However, due to their small size, current protocols for isolation of EVs are often time consuming, cumbersome, and require large sample volumes and expensive equipment, such as an ultracentrifuge. To address these limitations, we developed a paper-based immunoaffinity platform for separating subgroups of EVs that is easy, efficient, and requires sample volumes as low as 10 μl. Biological samples can be pipetted directly onto paper test zones that have been chemically modified with capture molecules that have high affinity to specific EV surface markers. We validate the assay by using scanning electron microscopy (SEM), paper-based enzyme-linked immunosorbent assays (P-ELISA), and transcriptome analysis. These paper-based devices will enable the study of EVs in the clinic and the research setting to help advance our understanding of EV functions in health and disease.
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