大肠杆菌
重组DNA
异源的
质粒
生物
异源表达
蛋白质表达
计算生物学
大肠杆菌蛋白质类
合成生物学
生物技术
遗传学
基因
作者
Germán L. Rosano,Eduardo A. Ceccarelli
标识
DOI:10.3389/fmicb.2014.00172
摘要
Escherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. For this reason, there are many molecular tools and protocols at hand for the high-level production of heterologous proteins, such as a vast catalog of expression plasmids, a great number of engineered strains and many cultivation strategies. We review the different approaches for the synthesis of recombinant proteins in E. coli and discuss recent progress in this ever-growing field.
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