Isolation of best tissue from Pleurotus eryngii and screening of optimal medium for mother mycelium culture

[Objective]The experiment was conducted to choose the optimal parts and culture medium type for optimal mother mycelium of Pleurotus eryngii in order to lay the foundation on its high-yield cultivation.[Method]About 0.5 cm2 internal tissue derived from the joint of pileus and stipe,and middle part and basal part of stipe of Pleurotus eryngii were taken and inoculated to PDA slant culture.The growing mycelia were purified and cultured for rejuvenation in 10 kinds of culture media.After rejuvenation,the mycelia were inoculated to wheat bran solid culture medium and soybean meal liquid medium to compare the growth of mycelia.Finally,the mycelia with best growth were inoculated to 10 kinds of media to screen the optimal culture medium.[Result]The growth of mycelium derived from middle part of Pleurotus eryngii stipe was significantly superior than those derived from other two parts,and the growth rate of mycelia derived from different parts differed significantly.The P.eryngii mother mycelium grew well in medium A(Potato),B(Cotton seed hull),C(Wheat bran),D(Wooden meal),E(Soybean) and G(Mung bean) with dense,neat,uniform color mycelium,but the growth rate of mycelium in medium C was the highest,followed by medium A and B.[Conclusion]The middle part of stipe was the best tissue for isolation of mother mycelium and the wheat bran juice medium was the appropriate culture medium for production of Pleurotus eryngii mother mycelium.