CD80
CD86
乳腺癌
质量细胞仪
单核细胞
流式细胞术
癌症研究
人口
生物
转移
树突状细胞
癌症
免疫学
病理
抗原
医学
T细胞
CD40
免疫系统
细胞毒性T细胞
生物化学
遗传学
环境卫生
基因
体外
表型
作者
Gabriel Valentín-Guillama,Fulya Üstün Alkan,Hilmi Alkan,Ahmad Alimadadi,Hasan Korkaya,Catherine C. Hedrick
出处
期刊:Journal of Immunology
[The American Association of Immunologists]
日期:2023-05-01
卷期号:210 (1_Supplement): 84.08-84.08
标识
DOI:10.4049/jimmunol.210.supp.84.08
摘要
Abstract Monocytes have emerged as important regulators of cancer progression. Our previous studies demonstrated the importance of nonclassical Ly6C lomonocytes in regulating cancer metastasis in mouse models. Here, we aimed to study monocyte heterogeneity in two different breast cancer tumors in mice using either 4T1 or EMT6 cells. 4T1 tumors exhibit an increased ability to metastasize in the lung in a BALB/c mouse breast cancer model compared to the less aggressive EMT6 tumor model. We evaluated 35 surface markers of tumor-infiltrated monocytes and dendritic cells in tumors isolated from each model using CyTOF mass cytometry. Analysis of monocytes and dendritic cells in the tumors using the Seurat package and the FlowSom algorithm identified 12 cell subsets. Significant differences were discovered between the two tumor types in frequencies of four of 12 identified subsets. However, only one of these subsets, a nonclassical monocyte subset, was higher in the less metastatic EMT6 tumors. This subset was 5-fold higher in EMT6 tumors versus more aggressive 4T1 tumors. This Ly6C lononclassical monocyte subset showed high expression of CD169 and CX3CR1, CD86 and CD80. These differentially expressed markers suggest a functional importance in this subset in migration to the tumor and antigen presentation, both of which are anti-tumoral functions. Ongoing studies are aimed towards understanding the role of this non-classical monocyte population in regulating breast cancer metastasis. This work was supported by NIH R01 CA202987, and P01 HL136275
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