染色质
免疫系统
转录组
溃疡性结肠炎
细胞外基质
生物
脱甲基酶
癌症研究
细胞外
干细胞
基因
结肠炎
免疫学
细胞生物学
基因表达
疾病
医学
遗传学
内科学
组蛋白
作者
Chuandong Liu,Jie Liu,Hua Jiang,Qian Zhao,Fangle Li,Zu-Rui Huang,Boyuan Mei,W. X. Gong,Fengchao Wang,Dali Han
标识
DOI:10.1093/lifemedi/lnad034
摘要
Abstract Ulcerative colitis (UC) is a chronic inflammatory disease of colon, which is characterized by crypt architectural distortion. Alternation of colonic stem cell (CoSC) contributed to the occurrence of UC, yet the regulatory mechanisms remain unclear. To investigate the dysregulation of transcriptional and post-transcriptional regulation, we performed RNA-seq, ATAC-seq, and m 6A meRIP-seq analysis of the cultured CoSCs that isolated from UC patients. The transcriptome analysis revealed distinct expression signatures of UC patients in mild and severe stages. We observed abnormal activation of immune and extracellular matrix-related genes in patients affected by severe UC. The chromatin accessibility at the promoter regions of these genes was also specifically increased in the severe stage. In addition, we identified that a global loss of RNA m 6A modification in severe stage accompanied by higher expression of the m 6A demethylase FTO. The aberrant activation of large number immune and extracellular matrix-related genes, including IL4R, HLA-DPA1 and COL6A1, was related to both gain of chromatin accessibility and loss of m 6A in severe UC patients. Our finding revealed an environment-independent immune activation of CoSCs in UC and provided FTO as a potential therapeutic target.
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