Bovine serum albumin-derived poly-l-glutamic acid-functionalized graphene quantum dots embedded UiO-66-NH2 MOFs as a fluorescence ‘On-Off-On’ magic gate for para-aminohippuric acid sensing

Evaluating para-aminohippuric acid (PAH) is emerging as a promising biomarker for the diagnostics of renal disease and other kidney-related illnesses. The present study aims to develop novel bovine serum albumin-derived poly-l-glutamic acid (PLGA) functionalized graphene quantum dots (PLGA-fGQDs) embedded in UiO-66-NH2 metal–organic frameworks ([email protected]2 MOFs) for monitoring of PAH. Initially, GQDs were achieved from bovine serum albumin (green precursor) via the single-step hydrothermal method. Here, functionalization with PLGA offers a tremendous increment in optical properties of GQDs. Then, highly luminescent UiO-66-NH2 MOFs were achieved using zirconium tetrachloride (ZrCl4) and 2-Aminoterephthalic acid (2-ATA) as a metal ion source and organic linker. Here, surface modification of GQDs with PLGA offered high quantum yield (QY), and responsiveness. Also, luminous UiO-66-NH2 MOFs afford a wide surface area for decorating of PLGA-fGQDs. The addition of gallium ions (Ga3+) into the probe solution resulted in fluorescence quenching (Turn-Off) whereas the incorporation of PAH resulted in fluorescence recovery (Turn-On). It is because of interaction with carboxylic functionality of PAH to Ga3+ followed by Ga-PAH complex formation. Herein, the wide concentration range and lowest limit of detection (LOD) were found to be 10 ng/mL to 900 ng/mL and 15.88 ng/mL, respectively. The specificity and real-time analysis in artificial urine validated the real-time adoption of a sensor for PAH detection. As well, it demonstrated good intraday/interday precision, stability analysis, and repeatability. In near future, the bundled illuminating [email protected]2 MOFs nanoprobe will be an attractive preference for tracking PAH in clinical specimens.