迷迭香酸
丹参
咖啡酸
纳米孔
原儿茶酸
传统医学
色谱法
化学
医学
生物化学
纳米技术
材料科学
抗氧化剂
替代医学
中医药
病理
作者
Pingping Fan,Shanyu Zhang,Yuqin Wang,Lijun Tian,Hanhan Zhang,Panke Zhang,Shuo Huang
标识
DOI:10.1038/s41467-024-45543-1
摘要
Natural herbs, which contain pharmacologically active compounds, have been used historically as medicines. Conventionally, the analysis of chemical components in herbal medicines requires time-consuming sample separation and state-of-the-art analytical instruments. Nanopore, a versatile single molecule sensor, might be suitable to identify bioactive compounds in natural herbs. Here, a phenylboronic acid appended Mycobacterium smegmatis porin A (MspA) nanopore is used as a sensor for herbal medicines. A variety of bioactive compounds based on salvianolic acids, including caffeic acid, protocatechuic acid, protocatechualdehyde, salvianic acid A, rosmarinic acid, lithospermic acid, salvianolic acid A and salvianolic acid B are identified. Using a custom machine learning algorithm, analyte identification is performed with an accuracy of 99.0%. This sensing principle is further used with natural herbs such as Salvia miltiorrhiza, Rosemary and Prunella vulgaris. No complex sample separation or purification is required and the sensing device is highly portable.
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