唾液腺
炎症
导管细胞
间充质干细胞
免疫系统
再生(生物学)
微泡
免疫学
生物
细胞生物学
医学
病理
癌症研究
免疫组织化学
小RNA
基因
生物化学
作者
Dong-Hyun Kim,Kyung Min Lim,Jaemin Cho,Hyojin Park,Seungyeon Hwang,Ahmed Abdal Dayem,Ye Jin Jeong,Yeokyung Shin,Hong Yan,Kwonwoo Song,Ssang-Goo Cho,Jae‐Yol Lim
标识
DOI:10.1016/j.jconrel.2023.03.055
摘要
Salivary gland dysfunction worsens the quality of life, but treatment for restoration of salivary gland function is limited. Although previous reports have demonstrated the therapeutic potentials of extracellular vesicles (EVs) in different preclinical models, the role of EVs in salivary glands remains elusive. Furthermore, little is known about the roles of salivary gland-derived EVs in tissue repair or regeneration compared to other EVs. In this study, EVs secreted from salivary gland-derived mesenchymal stem cells (sgMSCs) were comparatively analyzed with those from Wharton's jelly-derived MSC (wjMSCs). sgMSCs secreted more significant amounts of EVs than wjMSCs, and salivary gland epithelial cells showed a more efficient uptake of sgMSC-EVs than wjMSC-EVs. The possibility of immune regulation was tested via macrophage polarization and LPS-induced epithelial inflammation, resulting in an M1-to-M2 shift and reversal of acinar-to-ductal metaplasia by sgMSC-EV. Furthermore, the roles of sgMSC-EV-mediated immune regulation and tissue repair were clarified in vivo via retroductal delivery of sgMSC-EVs in a mouse model of obstructive sialadenitis. Collectively, our data demonstrate the superior role of sgMSC-EVs in the recovery from salivary gland inflammation and injury and suggest EVs as therapeutic tools for salivary gland dysfunction.
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