作者
Gary Reynolds,Péter Végh,James Fletcher,Elizabeth Poyner,Emily Stephenson,Issac Goh,Rachel A. Botting,Ni Huang,Bayanne Olabi,Anna Dubois,David Dixon,Kile Green,Daniel Maunder,Justin Engelbert,Mirjana Efremova,Krzysztof Polański,Laura Jardine,Claire Jones,Thomas Neß,Dave Horsfall,Jim McGrath,Christopher D. Carey,Dorin-Mirel Popescu,Simone Webb,Xiao-Nong Wang,Ben Sayer,Jong-Eun Park,Victor Augusti Negri,Daria Belokhvostova,Magnus Lynch,David McDonald,Andrew Filby,Tzachi Hagai,Kerstin B. Meyer,Akhtar Husain,Jonathan Coxhead,Roser Vento‐Tormo,Sam Behjati,Steven Lisgo,Alexandra‐Chloé Villani,Jaume Bacardit,Philip H. Jones,Edel A. O’Toole,Graham S. Ogg,Neil Rajan,Nick Reynolds,Sarah A. Teichmann,Fiona M. Watt,Muzlifah Haniffa
摘要
The skin confers biophysical and immunological protection through a complex cellular network established early in embryonic development. We profiled the transcriptomes of more than 500,000 single cells from developing human fetal skin, healthy adult skin, and adult skin with atopic dermatitis and psoriasis. We leveraged these datasets to compare cell states across development, homeostasis, and disease. Our analysis revealed an enrichment of innate immune cells in skin during the first trimester and clonal expansion of disease-associated lymphocytes in atopic dermatitis and psoriasis. We uncovered and validated in situ a reemergence of prenatal vascular endothelial cell and macrophage cellular programs in atopic dermatitis and psoriasis lesional skin. These data illustrate the dynamism of cutaneous immunity and provide opportunities for targeting pathological developmental programs in inflammatory skin diseases.