数字聚合酶链反应
2019年冠状病毒病(COVID-19)
严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)
金标准(测试)
实时聚合酶链反应
医学
病毒学
冠状病毒
切断
2019-20冠状病毒爆发
病毒
病毒载量
内科学
爆发
聚合酶链反应
疾病
生物
传染病(医学专业)
基因
物理
量子力学
生物化学
作者
Chong Liu,Qingxin Shi,Minfei Peng,Ruijie Lu,Haohao Li,Yingying Cai,Jiaxi Chen,Jiaqin Xu,Bo Shen
出处
期刊:Aging
[Impact Journals, LLC]
日期:2020-11-01
卷期号:12 (21): 20997-21003
被引量:26
标识
DOI:10.18632/aging.104020
摘要
The worldwide severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreak has led to the rapid spread of coronavirus disease (COVID-19). The quantitative real time PCR (qPCR) is widely used as the gold standard for clinical detection of SARS-CoV-2. However, more and more infected patients are relapsing after discharge, which suggests qPCR may fail to detect the virus in some cases. In this study, we selected 74 clinical samples from 43 recovering inpatients for qPCR and Droplet Digital PCR (ddPCR) synchronous blind detection, and established a cutoff value for ddPCR diagnosis of COVID-19. The results showed that at a cutoff value of 0.04 copies/μL, the ddPCR sensitivity and specificity are 97.6% and 100%, respectively. In addition, we also analyzed 18 retained samples from 9 discharged patients who relapsed. Although qPCR showed all 18 samples to be negative, ddPCR showed 12 to be positive, and there was only one patient with two negative samples; the other eight patients had at least one positive sample. These results indicate that ddPCR could significantly improve the accuracy of COVID-19 diagnosis, especially for discharged patients with a low viral load, and help to reduce misdiagnosis during recovery.
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