Adaptive super-resolution enabled on-chip contact microscopy

We demonstrate an adaptive super-resolution based contact imaging on a CMOS chip to achieve subcellular spatial resolution over a large field of view of ∼24 mm2. By using regular LED illumination, we acquire the single lower-resolution image of the objects placed approximate to the sensor with unit magnification. For the raw contact-mode lens-free image, the pixel size of the sensor chip limits the spatial resolution. We develop a hybrid supervised-unsupervised strategy to train a super-resolution network, circumventing the missing of in-situ ground truth, effectively recovering a much higher resolution image of the objects, permitting sub-micron spatial resolution to be achieved across the entire sensor chip active area. We demonstrate the success of this approach by imaging the proliferation dynamics of cells directly cultured on the chip.