In vitro adhezija S-proteina Lactobacillus sojeva na ekstracelularni matriks stanica crijevnog epitela

The aim of this work was to characterize ability of several S-layer-expressing Lactobacillus to adhere to the main proteins of the extracellular matrix (ECM). Four different Lactobacillus strains from the Collection of Lactic Acid Bacteria (ZBMK in Laboratory of Antibiotic, Enzyme, Probiotic and Starter Cultures Technology, Faculty of Food Technology and Biotechnology, over 200 strains), were used: Lactobacillus brevis ZG1-K7, Lactobacillus brevis SF9B; Lactobacillus paraplantarum SF15B and Lactobacillus plantarum D6. Four Lactobacillus pure cultures exhibited S-layer proteins according to the SDS-PAGE analyses. The binding capability of the Lactobacillus strains varied greatly among the isolates and was specific depending of ECM protein. Removal of the intact S-layer proteins from the bacterial surface by extraction with guanidine hydrochloride has reduced the adhesion of all of Lactobacillus strains to fibronectin and in most strains to laminin and collagen; whereas, the adhesiveness to laminin was increased, althought slightly, with L. brevis ZG1-K7 and L. paraplantarum SF15B, and to collagen with L. brevis SF9B and L. paraplantarum SF15B. Binding tests showed that mostly selected Lactobacillus strains adhere to ECM proteins in a proteinase K-sensitive manner.