生物
拟南芥
多细胞生物
转录组
计算生物学
细胞
基因
细胞命运测定
细胞分化
单细胞分析
基因表达
遗传学
细胞生物学
核糖核酸
转录因子
突变体
作者
Tian‐Qi Zhang,Zhou-Geng Xu,Guan-Dong Shang,Jia-Wei Wang
标识
DOI:10.1016/j.molp.2019.04.004
摘要
Cells of eukaryotic multicellular organisms have inherent heterogeneity. Recent advances in single-cell gene expression studies enable us to explore transcriptional regulation in dynamic development processes and highly heterogeneous cell populations. In this study, using a high-throughput single-cell RNA-sequencing assay, we found that the cells in Arabidopsis root are highly heterogeneous in their transcriptomes. A total of 24 putative cell clusters and the cluster-specific marker genes were identified. The spatial distribution and temporal ordering of the individual cells at different developmental stages illustrate their hierarchical structures and enable the reconstruction of continuous differentiation trajectory of root development. Moreover, we found that each root cell cluster exhibits distinct patterns of ion assimilation and hormonal responses. Collectively, our study reveals a high degree of heterogeneity of root cells and identifies the expression signatures of intermediate states during root cell differentiation at single-cell resolution. We also established a web server (http://wanglab.sippe.ac.cn/rootatlas/) to facilitate the use of the datasets generated in this study.
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