清脆的
Cas9
引导RNA
计算机科学
亚基因组mRNA
基因组编辑
计算生物学
基因组
可视化
生物
基因
遗传学
数据挖掘
作者
Jiamin Sun,Hao Líu,Jianxiao Liu,Shikun Cheng,Yong Peng,Qinghua Zhang,Jianbing Yan,Haijun Liu,Lingling Chen
出处
期刊:Bioinformatics
[Oxford University Press]
日期:2018-11-30
卷期号:35 (14): 2501-2503
被引量:28
标识
DOI:10.1093/bioinformatics/bty970
摘要
Abstract Summary CRISPR-Local is a high-throughput local tool for designing single-guide RNAs (sgRNAs) in plants and other organisms that factors in genetic variation and is optimized to generate genome-wide sgRNAs. CRISPR-Local outperforms other sgRNA design tools in the following respects: (i) designing sgRNAs suitable for non-reference varieties; (ii) screening for sgRNAs that are capable of simultaneously targeting multiple genes; (iii) saving computational resources by avoiding repeated calculations from multiple submissions and (iv) running offline, with both command-line and graphical user interface modes and the ability to export multiple formats for further batch analysis or visualization. We have applied CRISPR-Local to 71 public plant genomes, using both CRISPR/Cas9 and CRISPR/cpf1 systems. Availability and implementation CRISPR-Local can be freely downloaded from http://crispr.hzau.edu.cn/CRISPR-Local/. Supplementary information Supplementary data are available at Bioinformatics online.
科研通智能强力驱动
Strongly Powered by AbleSci AI