动力学
内化
合理设计
生物分子
荧光
纳米囊
多路复用
生物物理学
材料科学
荧光素酶
细胞
纳米技术
化学
生物
生物化学
生物信息学
纳米颗粒
量子力学
基因
物理
转染
作者
Di Wu,Yating Yang,Ping Xu,Duo Xu,Yang Liu,Roxanne Castillo,Ran Yan,Jie Ren,G.T. Zhou,Chaoyong Liu,Meng Qin,Juanjuan Du,Lihua Hou,Irvin S. Y. Chen,Chunsheng Kang,Lihua Jin,Jing Wen,Wei Chen,Yunfeng Lu
标识
DOI:10.1002/adma.201902469
摘要
Abstract Cells transport mass dynamically, crossing cell membranes to maintain metabolism and systemic homeostasis, through which biomolecules are also delivered to cells for gene editing, cell reprograming, therapy, and other purposes. Quantifying the translocation kinetics is fundamentally and clinically essential, but remains limited by fluorescence‐based technologies, which are semi‐quantitative and only provide kinetics information at cellular level or in discrete time. Herein, a real‐time method of quantifying cell internalization kinetics is reported using functionalized firefly‐luciferase nanocapsules as the probe. This quantitative assay will facilitate the rational design of delivery vectors and enable high‐throughput screening of peptides and other functional molecules, constituting an effective tool for broad applications, including drug development and cancer therapy.
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