Curcumin Loaded onto Folic acid Carbon dots as a Potent drug Delivery System for Antibacterial and Anticancer Applications

Abstract Numerous malignancies have been shown to be successfully treated with Curcumin. Despite its promising effects, Curcumin has limitations in clinical studies because of its stability, low water solubility, and adsorption. Carbon quantum dots with high biocompatibility can be employed as nanostructured material carriers to enhance Curcumin availability. In this study, folic acid was used as the raw material for the hydrothermal preparation of carbon dots, followed by the loading of Curcumin onto the carbon dots to form a folic acid carbon dot/Curcumin nanocomposite. The morphology and the chemical structure of the synthesized carbon dots were investigated. Folic acid carbon dots displayed robust emission peaks with a quantum yield of 41.8%. Moreover, the adsorption effectiveness of Curcumin on carbon dots was determined to be 83.11%. The liberating pattern of Curcumin was pH-dependent and reached 36 and 27% after a few hours at pH 5 and 7.4, respectively. The release occurs via the Fickiann diffusion mechanism with ah n value less than 0.45.The nanocomposite was tested for antibacterial activity against gram-negative Pseudomonas aeruginosa ATCC 27,853 and gram-positive Staphylococcus aureus ATCC 25,923 . The nanocomposite displayed antibacterial behavior with MIC 12.5 µg/mL. The anticancer activities of the nanocomposite were further tested against high-folate receptor-expressing Hela cells (cervical malignancy) and low-folate receptor-expressing HepG2 cells (hepatocellular carcinoma). Folic acid carbon dot/Curcumin nanocomposite reduced Hela cell viability at an IC 50 of 88.723 ± 0.534 g/mL. On the other hand, HepG2 cells showed no toxicity response.